To assess whether the transient alopecia was caused by loss of a keratinocyte-intrinsic function of IKKε and TBK1, we generated IkkeK38A/K38ATbk1fl/D135NK14-Crewt/tg mice that lack TBK1 kinase activity specifically in keratinocytes and IKKε kinase activity in all cells. This evidence concerns the gene IKBKE and alopecia.