Furthermore, we depicted the schematic diagram of the current research as shown in Fig. 7C. Shikonin activates the expression of GADD45B and PPP3CC through the JNK/P38/MAPK pathway to suppress the growth and migration abilities of NSCLC cells, providing an alternative mechanism for the inhibitory function of shikonin on NSCLC. This evidence concerns the gene GADD45B and non-small cell lung carcinoma.