Furthermore, we depicted the schematic diagram of the current research as shown in Fig. 7C. Shikonin activates the expression of GADD45B and PPP3CC through the JNK/P38/MAPK pathway to suppress the growth and migration abilities of NSCLC cells, providing an alternative mechanism for the inhibitory function of shikonin on NSCLC. The gene discussed is MAPK8; the disease is non-small cell lung carcinoma.