We selected two lung adenocarcinoma cell lines to verify the expression of the hub gene, and found that CSMD3, LRP1B, MUC16, TTN were highly expressed in lung adenocarcinoma cells, which provided effective evidence to support that they play a vital role in the development of lung adenocarcinoma and may be a new therapeutic target, which deserves more attention in follow-up research. Here, MUC16 is linked to lung adenocarcinoma.