Our data showed that mycobacterium tuberculosis (Mtb)-specific IFN-γ, TNF-α, IL-2, IL-6, IL-10, IL-5, IP-10, IL-1Ra, CXCL-1 and MCP-1 responses based on QFT-Plus significantly differed between Mtb-infected (including ATB, LTBI and previous TB) and uninfected healthy populations. The gene discussed is TNF; the disease is tuberculosis.