PCLO and glioblastoma: Since, our study aimed to identify the most commonly mutated genes in GBM patients, we examined the top 100 most frequently mutated genes and identified ten genes that were commonly altered across all datasets, including PTEN, TP53, TTN, MUC16, FLG, PCLO, MUC17, HMCN1, AHNAK2, and OBSCN (Fig 2A).