Western blot analysis of the expression of autophagy-related proteins in tissues showed that compared with the NC group, the expression of p62 protein in the DM group was significantly upregulated, the expression of LC3 II/I and Beclin 1 was significantly downregulated, and the expression of p62 protein was significantly downregulated after overexpression of Sestrin2. This evidence concerns the gene BECN1 and diabetes mellitus.