In some samples we also identified several EV proteins that were previously suggested to be liquid biopsy markers for lung (LRG1 (Leucine-rich alpha-2-glycoprotein), tetraspanin-8), prostate (PSA (prostate-specific antigen)), bladder (TACD2 (Tumor-associated calcium signal transducer 2)), colorectal and pancreatic (glypican-1) cancers (reviewed by 46) (Figure 3B). This evidence concerns the gene TSPAN8 and cancer.