Besides, although the mechanisms of osteogenesis during DO and other bone regeneration models are not the same because of the rhythmic mechanical stimuli in DO, FAK signaling is also important to osteogenesis in fracture 46, osteoporosis 47, etc. While we primarily focused on the effect of ZN27 on DO in the present study, the potential effect of ZN27 in other bone regeneration models could be further explored. Here, PTK2 is linked to osteoporosis.