To determine the role of SECTM1 in GBM, we constructed shRNAs targeting SECTM1 (SECTM1-sh1, SECTM1-sh2) and negative control shNC, knocked down the expression of SECTM1 in GBM cells (U251 MG and U87 MG cells), RT-qPCR and immunoblot analysis detected knockdown effects (Figures 3A-C, E-G). Here, SECTM1 is linked to glioblastoma.