PD-L1 mRNA expression was inhibited by 36.5% (CGA +Anti-PD-1 vs. NS + Anti-PD-1, p < 0.05) in the MC38 tumor tissues and 31.0% (CGA +Anti-PD-1 vs. NS + Anti-PD-1, p < 0.01) in the 4T1 tumor tissues. The gene discussed is PDCD1; the disease is neoplasm.