In this study, 7 cancer cells (5 human cancer cell lines and 2 murine cancer cell lines) were agitated by IFN-γ and then treated with CGA; among the 7 cell lines human melanoma (A375), ovarian cancer (SK-OV-3), and triple-negative breast cancer (MDA-MB-231) cells were reported to have basic PD-L1 expression. The gene discussed is CD274; the disease is melanoma.