And the PD-L1 protein expression was decreased by 30.5% (CGA +Anti-PD-1 vs. NS + Anti-PD-1, p < 0.05) and 18.9% (CGA +Anti-PD-1 vs. NS + Anti-PD-1, p < 0.01) in the MC38 and 4T1 tumor tissues, respectively. This evidence concerns the gene PDCD1 and neoplasm.