Furthermore, KEGG enrichment analysis with all differential genes showed that the p53 signaling pathway was the most significantly enriched pathway in the mRNA-seq (Fig. 3E), and the differential genes were also enriched in various other pathways including base excision repair, parathyroid hormone synthesis and secretion, amyotrophic lateral sclerosis, herpes simplex virus 1 infection, JAK-STAT signaling pathway, transcriptional misregulation in cancer, ABC transporters, phenylalanine metabolism, and human T-cell leukemia virus 1 infection (Fig. 3E). This evidence concerns the gene TP53 and amyotrophic lateral sclerosis.