However, for the tumor-bearing mice group, the NIR-II signals showed distinct enhancement in Ch.1 at 8 h post-injection (p.i.), and exhibited a very weak increase in Ch.2, receiving a distinct ratiometric signal variation, thus indicating that the endogenous H2S generated by liver tumor cells could be effectively detected by FRHS (Fig. 5E). The gene discussed is SUCO; the disease is neoplasm.