To test this hypothesis, we combined electrophysiological recording, behavioral test, optogenetics manipulation and SNS activity analyses, and found there is a negative correlation between anxiety-like behavior and stress-hormone in peripheral serum, including NE, cortisol and renin, which represented activity of SNS (Hu et al., 2020), HPA (Young et al., 2021) and RAAS (Renin-angiotensin-aldosterone system) (Ames et al., 2019), respectively. The gene discussed is REN; the disease is Anxiety.