We can conclude that total LH determinations by LIA in FMV urine samples can be used to evaluate pubertal development in patients with thyroid pathology as an alternative to the discontinued IFMA assays and as a general alternative to invasive serum assays, provided that the patient is in a euthyroid state and has not been on levothyroxine treatment for at least three months prior to sampling. The gene discussed is PLOD1; the disease is thyroiditis.