Phospho-mimetic and phospho-null constructs were generated by mutating Ser/Thr/Tyr to Glu (E) or Ala (A), respectively, to study these phosphorylation sites in the presence of AD tau core 306–378 at 12 sites in the PRR (designated 12E and 12A) and 9 sites in the C terminus (designated 9E and 9A) (Figure 7A). Here, MAPT is linked to Alzheimer disease.