Studies have shown that acupuncture points such as HT7, SP6, and GV20 can regulate neurotransmitters such as 5-hydroxytryptamine, norepinephrine, dopamine, γ-aminobutyric acid, glutamate, acetylcholine, and orexin, to adjust the structure of sleep and improve the state of anxiety and depression (45–47). This evidence concerns the gene HCRT and major depressive disorder.