PEGylated hybrid-NPs system encompassed calcium phosphate/liposomes co-loaded with NF-κB specific siRNA and MTX were developed to target macrophages, aiming to inhibit p65 and its translocation.312 In-vivo results demonstrated that the liposomal formulation could retard the RA progression by preventing the release of pro-inflammatory cytokines from macrophages without affecting the lymphocyte count, which could prevent the adverse effect of MTX. This evidence concerns the gene NFKB1 and rheumatoid arthritis.