A total of 16 cuproptosis regulators (FDX1, DLD, LIAS, LIPT1, DLST, DLAT, DBT, GCSH, PDHA1, ATP7A, ATP7B, PDHB, SLC31A1, MTF1, GLS, CDKN2A) were screened according to difference analysis between the control and IDD groups. This evidence concerns the gene DLST and intervertebral disk degenerative disorder.