A total of 16 cuproptosis regulators (FDX1, DLD, LIAS, LIPT1, DLST, DLAT, DBT, GCSH, PDHA1, ATP7A, ATP7B, PDHB, SLC31A1, MTF1, GLS, CDKN2A) were screened according to difference analysis between the control and IDD groups. The gene discussed is CDKN2A; the disease is intervertebral disk degenerative disorder.