CTSB and retinoblastoma: In addition, Western blot analysis of autophagy-related protein expression showed that the levels of LC3II/I and Beclin1 were prominently elevated and that the level of p62 was markedly decreased in the OE-CTSB group compared to the RB group, while the opposite trends in terms of changes in protein expression were observed in the si-CTSB group (Fig. 2D).