To examine the effect of CTSB on autophagy-dependent death in RB cells, first, the transfection efficiency of CTSB was examined, and the results showed that, compared with that in the RB group, CTSB expression was significantly upregulated after overexpression of CTSB (OE-CTSB) and significantly downregulated after knockdown of CTSB (si-CTSB) (Fig. 2A), suggesting that the transfection of the OE-CTSB and si-CTSB constructs was successful. The gene discussed is CTSB; the disease is retinoblastoma.