Participants were asked to self-assess the presence/absence of male-like hair patterns and menstrual irregularity and were then invited to undergo a direct examination, including completing a medical history and modified Ferriman-Gallwey hirsutism score, ovarian ultrasound, and measurement of circulating total and free testosterone, DHEAS, TSH, prolactin, and 17-hydroxyprogesterone levels [39]. This evidence concerns the gene PRL and Hirsutism.