Next, we performed a flow cytometry assay to further explore the relationship between improved NAFLD symptoms and macrophages, finding significant differences in the number of macrophages between the INOS−/− and INOS+/+ mice; specifically, the INOS−/− group had significantly fewer macrophages than the INOS+/+ group (Figure 4A–C). This evidence concerns the gene NOS2 and metabolic dysfunction-associated steatotic liver disease.