Our results suggest that lnc‐ANRIL is involved in the regulation of cell proliferation, cell cycle, and cell apoptosis of MAC‐T cells, and plays an important role in the inflammatory and immune response of MAC‐T cells through the regulation of the NF‐κB pathway, proposing new therapeutic strategies for the treatment of innate immune response‐related disease such as bovine mastitis. Here, NFKB1 is linked to Bovine mastitis.