To validate the DEGs and identify their association with cellular dysregulation by RPL8-OE in HeLa cells, RT-qPCR was performed using several randomly selected DEGs from the cancer-related pathways, including AVPR2, DLL4, IL23A, FGF1, ACSF2, C8G, FCGR2A and FOS. The gene discussed is AVPR2; the disease is cancer.