It was consistent with a previous study that reported BDNF release within the mPFC is necessary for the antidepressant actions of ketamine.[57] Moreover, in this study, supplementation of BDNF in the dLS or overexpression of AAV‐BDNF in the LC could significantly alleviate the depression‐like behaviors caused by CSDS. This evidence concerns the gene BDNF and major depressive disorder.