Compared to chow-fed controls, liver tumors in GAN DIO-NASH-HCC mice demonstrated distinct enrichment of activated genes associated with human HCC subclass S1 (Wnt/TGFβ-proliferation, Fig. 3B), which has been associated with poor prognosis in HCC patients34. The gene discussed is TGFB1; the disease is metabolic dysfunction-associated steatohepatitis.