In addition to well-demarcated tumor appearance, cytological atypia (mild nuclear pleomorphism, increased nuclear-to-cytoplasmic ratio) and extensive depletion of reticulin trabecular framework, also reported previously in GAN DIO-NASH-HCC mice20, tumors displayed abnormal glutamine synthetase staining. The gene discussed is GLUL; the disease is metabolic dysfunction-associated steatohepatitis.