Progressive NASH and fibrosis in GAN DIO-NASH-HCC mice was supported by quantitative histomorphometric analysis, indicating progressive increases in lipid accumulation (HE staining), inflammation (galectin-3), fibrogenic cell activity (α-SMA) and collagen deposition (PSR staining, Col1a1) (Figs. 1K–O, S1). This evidence concerns the gene ACTA1 and hepatocellular carcinoma.