To further investigate FOXD2 function for proper renal development and to understand how biallelic FOXD2 dysfunction may cause bilateral renal hypodysplasia/CAKUT in humans and mice, Foxd2-deficient immortalized mouse metanephric cell models (mk4 cells) were generated using CRISPR/Cas9 technology. This evidence concerns the gene FOXD2 and congenital anomaly of kidney and urinary tract.