KEGG analysis showed that CCR7 was enriched in the cytokine-cytokine receptor interaction, JAK2 was enriched in the hepatitis B and JAK-STAT signaling pathway, and TLR4 was enriched in the hepatitis B. Therefore, we speculated that CCR7, JAK2 and TLR4 may play a role in CIS by regulating related pathways. This evidence concerns the gene TLR4 and in situ carcinoma.