GNB4 had a high sensitivity of 83.82% for any-stage HCC detection, but a relatively low specificity of 87.54% in CLD and healthy cohorts; Riplet had a relatively low sensitivity of 66.47% for any-stage HCC detection, but a high specificity of 97.98% in CLD and healthy cohorts. The gene discussed is GNB4; the disease is hepatocellular carcinoma.