CD3D and cancer: We used unsupervised clustering and 2D embedding using uniform manifold approximation and projection (UMAP) as well as a marker-based annotation strategy to identify 6 major cell types: tumor cells (Epcam+), B cells (Cd79a+), T cells (Cd3d+), myeloid cells (Itgam+), cancer associated fibroblasts (CAFs) (Dcn+), and endothelial cells (Pecam1+) (Figure 1, C and D).