Diosgenin, one of the primary bioactive compounds found in fenugreek seeds (Arya and Kumar, 2021), shows promise in rectifying gut microbiota imbalances, regulating HPA axis secretion levels, upregulating hippocampal BDNF signaling pathway expression, promoting AHN, and treating depression (Cui et al., 2023). This evidence concerns the gene BDNF and depressive symptom measurement.