Importantly, overexpression of GCH1 significantly rescued BHPF-mediated downregulation of BH2 (Supplementary Fig. 3J), and repressed BHPF-induced upregulation of ferroptosis and heart failure biomarkers (Supplementary Fig. 3K), which was accompanied by relieving of BHPF-induced reduction in heart rate and cardiac viability (Fig. 2H and I). This evidence concerns the gene GCH1 and heart failure.