Here, we tested the usefulness of dynamic clamp in detecting DADs in hiPSC-CMs generated from two patients who are homozygous or compound heterozygous for recessive mutations in the ACADVL gene that result in mitochondrial long-chain fatty acid oxidation (lcFAO) dysfunction and are associated with cardiac arrhythmias. The gene discussed is ACADVL; the disease is chronic obstructive pulmonary disease.