However, the IFN-γ secretion by CD4+ T cells of the rEg.P29+CpG+Infection group with rEg.P29 stimulation showed no difference compared with that without stimulation, and the number of CD4+ T cells was not different compared with that of the PBS+Infection group and CpG+Infection group (Figure 4A‒D). This evidence concerns the gene CD4 and infection.