To determine the sensitivity and mitotic effects caused by KIF18A loss, we compared the impact of KIF18A knockdown (KD) versus non-targeting control (NTC) or essential mitotic kinesin KIF11 (also known as EG5) small interfering RNA (siRNA) species on a panel of human breast and ovarian cancer cell lines as well as normal human mammary epithelial cells (HMECs) (Fig. 1a and Extended Data Fig. 1a). The gene discussed is KIF18A; the disease is ovarian carcinoma.