However, an alternative study using a large-scale tissue microarray showed independent co-expression of SFRP4 and p53, and the absence of SFRP4 expression was associated with the negative expression of COX-2 and MLH1. Therefore, the overexpression of SFRP4 in CRC remains unclear, so that SFRP4 might have a role at the intersection of the Wnt pathway and other signaling pathways46. This evidence concerns the gene SFRP4 and colorectal carcinoma.