SHISA8 and neoplasm: We next carried out qRT-PCR analysis of expression of seven ‘inflammation and immunity’-/‘NFκB’-associated genes (Bcl2a1a, Cd40, Nfkb2, Relb, Ccl5, Tnfaip3 and Shisa8) differentially expressed between the Lyn groups and PCA groups of tumours using twenty of the samples (Table S12) analysed by RNAseq in order to validate the analysis.