LYN and neoplasm: To understand more objectively the relationship between tumour genotype and LYN expression in tumour cells, in the absence of cells from the tumour microenvironment that might also express LYN, we isolated live cells from six tumours of each cohort (Lynwt/wt, Lynfl/wt and Lynfl/fl) and cultured them in conditions optimised for primary culture of epithelial tumour cells, before harvesting DNA, RNA and protein for analysis (DNA was only available for analysis from five of the six Lynfl/wt samples).