To validate the expression of these genes at single-cell level, visualize their spatial distribution within complex tissue structures and validate co-expression patterns, functional gene interactions, we performed RNA fluorescence in situ hybridization (FISH) on paraffin sections of PDAC tumor tissue (Figure 11B).Our analysis revealed frequent overlap of the GLUL, SQSTM1 and S100A4, providing evidence for the existence of the GLUL+SQSTM1+ RTM. This evidence concerns the gene S100A4 and neoplasm.