To identify the involvement of Casp3-dependent apoptosis in neutrophils during Bb infection, BMNs were infected with Bb at 10 and 100 MOI and the Casp3 activity in BMNs was determined at 1 hpi using green-fluorescent YO-PROTM-1 (YP-1) Iodide staining, and flow cytometry was performed (Figure 5B). The gene discussed is CASP3; the disease is infection.