EPCAM and cancer: In detail, these CAR-Ts mediated effective antitumor activity and produced INF-γ when co-cultured with different EpCAM-expressing cancer cell lines, including 4T1 and MC38; however, they did not demonstrate cytotoxicity against the EpCAM-deficient normal fibroblast control cells NIH-3T3, indicating the antigen-specific tumoricidal activity of these effector cells (85).