In a previous study, we performed knockdown-rescue experiments to demonstrate that Pfn1 depletion in MDA-MB-231 (referred to as MDA-231 hereon) breast cancer cells prominently enhances PM accumulation of PI(3,4)P2 downstream of activated RTK (EGFR and PDGFR) without impacting RTK activation (23). This evidence concerns the gene PFN1 and breast carcinoma.