In a previous study, we performed knockdown-rescue experiments to demonstrate that Pfn1 depletion in MDA-MB-231 (referred to as MDA-231 hereon) breast cancer cells prominently enhances PM accumulation of PI(3,4)P2 downstream of activated RTK (EGFR and PDGFR) without impacting RTK activation (23). Here, PFN1 is linked to breast cancer.