AKT1 and cardiovascular disorder: According to the results of GO annotation analysis, KEGG pathway analysis and PPI protein interaction analysis, three proteins (Ppp1cc: Insulin signaling pathway, cAMP signaling pathway, vascular smooth muscle contraction and others; Gys1: Pi3k/Akt signaling pathway, Eef2: AMPK signaling pathway), which were located in the center of the PPI interaction map and significantly related to cardiovascular disease pathways, were selected for western blot verification.