According to the results of GO annotation analysis, KEGG pathway analysis and PPI protein interaction analysis, three proteins (Ppp1cc: Insulin signaling pathway, cAMP signaling pathway, vascular smooth muscle contraction and others; Gys1: Pi3k/Akt signaling pathway, Eef2: AMPK signaling pathway), which were located in the center of the PPI interaction map and significantly related to cardiovascular disease pathways, were selected for western blot verification. The gene discussed is INS; the disease is cardiovascular disorder.