Stress was induced in 42 BALB/c mice using a conventional restraint model. The mRNA expression of neurokinin 1 receptor (NK1R) and the NK1R protein expression were increased in animals given anxiety as compared to the normal control. Both parameters decreased in animals treated with eugenol and its liposome-based nanocarriers, the results being better for nanocarriers. Here, TACR1 is linked to Anxiety.