For such purposes, the aim of this study was to use the HCT116 WT colon cancer cell line for genome editing using the CRISPR/Cas-9 technique in order to knock-out the glutathione peroxidase 4 (GPX4) gene, and to investigate how this process would affect the induction of regulated cell death (ferroptosis) and other cellular processes (Figure 1). The gene discussed is GPX4; the disease is malignant colon neoplasm.