HMGB1 and hepatocellular carcinoma: In a recent in vitro study on thermal ablation, researchers analyzed the changes in the concentrations of ICD-related cytokines produced by different HCC tumors at different temperatures, including ATP, HMGB1, and CXCL10, and the results indicated that extremely low temperatures (−80 °C) and higher temperatures (60 °C) are more conducive to facilitating the ICD effect [57].