In dextran sulfate sodium (DSS)-induced colitis, splenic NKG2D+CD4+ T cells could be divided into two subpopulations based on the expression of NK1.1, namely TGF-β+FasL+T-bet+NK1.1− cells and IFN-γ+IL-17+IL21+granzymeB+perforin+T-bet−RORγt+NK1.1+ cells. Here, CD4 is linked to colitis.