We identified six miRNAs—let-7f-5p (in LGMDR1), miR-20a-5p (in LGMDR2), miR-130b-5p, miR-378a-5p (both in LGMDR3), miR-376c-3p and miR-382-5p (both in LGMDR4)—whose expressions were significantly lower compared to the controls in the different LGMD pools. Here, CAPN3 is linked to limb-girdle muscular dystrophy.