The effect of IFN-γ on NSCLC cells is dose-dependent: low-dose IFN-γ induces the EMT and enhances the stemness of A549 and H460 cells both In vitro and in vivo by activating the intercellular adhesion molecule 1 (ICAM1)/PI3K/Akt/Notch1 signaling axis; high-dose IFN-γ induces apoptosis of NSCLC cells through the JAK1/STAT1/caspase-3,7 pathway. This evidence concerns the gene AKT1 and non-small cell lung carcinoma.