GFAP and Hyperammonemia: Hyperammonemia also increased the activation of astrocytes, reflected in an increase in the area covered by the anti-GFAP staining (23 ± 1.6%; p < 0.05) compared to the control rats (16 ± 1.2) (Figure 4b,d), while JTE-013 reversed the astrocytes activation (12 ± 4.3%, p < 0.01) (Figure 4b,d).