IGHE and granulomatosis with polyangiitis: There were six objectives for this research: (1) establish a colony of plant protein-free Balb/c mice colony; (2) Assess WG’s inherent sensitization potential (i.e., IgE response) via repeated skin application; (3) investigate anaphylactic clinical symptoms upon systemic WG challenge; (4) Quantify the anaphylaxis using hypothermic shock responses (HSR); (5) Measure mucosal mast cell degranulation responses (MMCR) by quantifying blood levels of MMCP-1; and (6) Identify spleen biomarkers associated with life-threatening systemic anaphylaxis in this model.