IGHE and anaphylaxis: The limitations of the study include: (i) indicators of anaphylaxis, such as clinical symptom scores, hypothermic shock responses, histamine responses, or mucosal mast cell mediator proteins were not reported; therefore, whether IgE antibody response resulted in clinical sensitization for systemic anaphylaxis was not studied; (ii) this was a complete Freund’s adjuvant-based model to elicit sensitization; therefore, intrinsic sensitization capacity of wheat glutenin was not studied.