Of the fifteen patients with positive results, seven were diagnosed with bilateral retinoblastoma and had a P/LP variant in RB1; three with ALL had a P/LP variant in PALB2, VHL, and MUTYH; one with recurrent desmoid tumor had a P/LP variant in FANCM; one with recurrent astrocytoma had a P/LP variant in P53 gene; and three with solid tumors had the following P/LP variants: one with optic glioma had a P/LP variant in NF1; one with Ewings sarcoma had a P/LP variant in BAP1; and one with neuroblastoma had a P/LP variant in SMARCA4. This evidence concerns the gene VHL and astrocytoma (excluding glioblastoma).